Is Your Cell Culture Mycoplasma Free? How Confident Are You?
Mycoplasmas, the smallest known free living organisms, are relatively common bacterial contaminants of mammalian cell cultures. For major pharmaceutical companies in Berkeley, Oakland and the Bay Area, regulatory guidelines require that all products derived from mammalian cell culture are tested for the presence of Mycoplasma. However, the traditional testing by direct inoculation and growth of Mycoplasma species on agar or broth media requires up to 28 days. Regardless of whether you are a basic scientist performing routine research or a major pharmaceutical company engaged in cell culture work, one may argue that mycoplasma testing is expensive, time consuming, and complicated. [Read more…]